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Copyright © 2005-2006 Environmental Technical Group, Inc.
Advanced Water
What is E. coli and where does it come from?
E. coli is a type of fecal coliform bacteria commonly found in the intestines of animals and humans.  E.
coli is short for Escherichia coli.  The presence of E. coli in water is a strong indication of recent
sewage or animal waste contamination. Sewage may contain many types of disease-causing

What are fecal coliforms?
Fecal coliforms are bacteria that are associated with human or animal wastes.  They usually live in
human or animal intestinal tracts, and their presence in drinking water is a strong indication of recent
sewage or animal waste contamination.

How does E. coli or other fecal coliforms get in the water?
E. coli comes from human and animal wastes.  During rainfalls, snow melts, or other types of
precipitation, E. coli may be washed into creeks, rivers, streams, lakes, or groundwater.  When these
waters are used as sources of drinking water and the water is not treated or inadequately treated, E.
coli may end up in drinking water.

What are the health effects of E. coli O157:H7?
E. coli O157:H7 is one of hundreds of strains of the bacterium E. coli.  Although most strains are
harmless and live in the intestines of healthy humans and animals, this strain produces a powerful
toxin and can cause severe illness.  Infection often causes severe bloody diarrhea and abdominal
cramps; sometimes the infection causes non-bloody diarrhea.  Frequently, no fever is present.  It
should be noted that these symptoms are common to a variety of diseases, and may be caused by
sources other than contaminated drinking water.

In some people, particularly children under 5 years of age and the elderly, the infection can also
cause a complication called hemolytic uremic syndrome, in which the red blood cells are destroyed
and the kidneys fail. About 2%-7% of infections lead to this complication. In the United States,
hemolytic uremic syndrome is the principal cause of acute kidney failure in children, and most cases
of hemolytic uremic syndrome are caused by E. coli O157:H7. Hemolytic uremic syndrome is a life-
threatening condition usually treated in an intensive care unit. Blood transfusions and kidney dialysis
are often required. With intensive care, the death rate for hemolytic uremic syndrome is 3%-5%.

How long does it take for these symptoms of E. coli O157:H7 infection to occur?
Symptoms usually appear within 2 to 4 days, but can take up to 8 days.  Most people recover without
antibiotics or other specific treatment in 5-10 days. There is no evidence that antibiotics improve the
course of disease, and it is thought that treatment with some antibiotics may precipitate kidney
complications. Antidiarrheal agents, such as loperamide (Imodium), should also be avoided.

What should I do if I have any of the above symptoms?
Consult with your physician.  Infection withE. coli O157:H7 is diagnosed by detecting the bacterium in
the stool. Most laboratories that culture stool do not test for E. coli O157:H7, so it is important to
request that the stool specimen be tested on sorbitol-MacConkey (SMAC) agar for this organism. All
persons who suddenly have diarrhea with blood should get their stool tested for E. coli O157:H7.

Are there groups of people who are at greater risk of getting any of the symptoms?
Children under the age of five, the elderly, and people whose health is weakened (i.e., people who
have long-term illnesses such as cancer or AIDS) are at greater risk of severe illness.

What should these people who are at greater risk do?  Are there any additional precautions
they should take?
People who are at greater risk should consult with their doctor or health care provider and follow the
instructions provided.

How will I know if my water is safe?
If you get your water from a public water system, then your water system is required by law to notify
you if your water is not safe.  If you are interested in obtaining information about your drinking water,
consult the water quality report that you should receive annually from your local water system, or call
your local water system directly.  

How is water treated to protect me from E. coli?
The water can be treated using chlorine, ultra-violet light, or ozone, all of which act to kill or
inactivateE. coli.  Systems using surface water sources are required to disinfect to ensure that all
bacterial contamination is inactivated, such as E. coli.  Systems using ground water sources are not
required to disinfect, although many of them do.

If I have a private well, how can I have it tested for E. coli?
If you have a private well, you should have your water tested periodically.  Contact your State
laboratory certification officer to find out which laboratories have been certified for conducting total
coliform analyses.  (You may contact the Safe Drinking Water Hotline at 1-800-426-4791 for the
address and phone number of this individual.)  Then contact a certified lab near you and get
instructions on how to send them a water sample.  Typically, the lab will first test for total coliforms,
which is a group of related organisms that is common in both the environment and in the gut of
animals.  If the sample is positive for total coliforms, the lab will determine whether E. coli is also
present.  E. coli is a type of total coliform that is closely associated with recent fecal contamination.  
Few E. coli strains cause disease.  However, the presence of any E. coli in a water sample suggests
that disease-causing organisms, are also likely to be present.

One of the strains of E. coli that causes disease is E. coli O157:H7.  EPA does not believe it
necessary for an owner of a private well to test specifically for this organism under normal
circumstances.  If E. coli O157:H7 is present in your well, it is highly likely that other strains of E. coli
are also present.  If a well is E. coli-positive, regardless of strain, you should not drink the water
unless it is disinfected.  Several tests are available for determining whether E. coli O157:H7 is
present, but they are somewhat more expensive than the standard E. coli tests and many labs may
not have the expertise or supplies to perform these tests.  Your state's laboratory certification officer
should be able to tell you which laboratories can perform these tests, or you can contact the lab

If my well is contaminated with E. coli, what can I do to protect myself?
If your well tests positive for E. coli , do not drink the water unless you boil it for at least one minute at
a rolling boil, longer if you live at high altitudes.  You may also disinfect the well according to
procedures recommended by your local health department.  Monitor your water periodically after
disinfection to make certain that the problem does not recur.  If the contamination is a recurring
problem, you should investigate the feasibility of drilling a new well or install a point-of-entry
disinfection unit, which can use chlorine, ultraviolet light, or ozone.

How does the U.S. Environmental Protection Agency regulate E. coli?
According to EPA regulations, a system that operates at least 60 days per year, and serves 25
people or more or has 15 or more service connections, is regulated as a public water system under
the Safe Drinking Water Act.  If a system is not a public water system as defined by EPA's regulations,
it is not regulated under the Safe Drinking Water Act, although it may be regulated by state or local

Under the Safe Drinking Water Act, EPA requires public water systems to monitor for coliform
bacteria.  Systems analyze first for total coliform, because this test is faster to produce results.  Any
time that a sample is positive for total coliform, the same sample must be analyzed for either fecal
coliform or E. coli.  Both are indicators of contamination with animal waste or human sewage.

The largest public water systems (serving millions of people) must take at least 480 samples per
month.  Smaller systems must take at least five samples a month unless the state has conducted a
sanitary survey – a survey in which a state inspector examines system components and ensures they
will protect public health – at the system within the last five years.

Systems serving 25 to 1,000 people typically take one sample per month.  Some states reduce this
frequency to quarterly for ground water systems if a recent sanitary survey shows that the system is
free of sanitary defects.  Some types of systems can qualify for annual monitoring.

Systems using surface water, rather than ground water, are required to take extra steps to protect
against bacterial contamination because surface water sources are more vulnerable to such
contamination.  At a minimum, all systems using surface waters must disinfect.  Disinfection will kill E.
coli O157:H7.

What can I do to protect myself from E. coli O157:H7 in drinking water?
Approximately 89 percent of Americans are receiving water from community water systems that meet
all health-based standards.  Your public water system is required to notify you if, for any reason, your
drinking water is not safe.  If you wish to take extra precautions, you can boil your water for one
minute at a rolling boil, longer at higher altitudes.  To find out more information about your water, see
the Consumer Confidence Report from your local water supplier or contact your local water supplier
directly.  You can also obtain information about your local water system on EPA's web site at www.epa.

If you draw water from a private well, you can contact your state health department to obtain
information on how to have your well tested for total coliforms and E. coli contamination.  If your well
tests positive for E. coli, there are several steps that you should take: (1) begin boiling all water
intended for consumption, (2) disinfect the well according to procedures recommended by your local
health department, and (3) monitor your water quality to make certain that the problem does not
recur.  If the contamination is a recurring problem, you should investigate the feasibility of drilling a
new well or install a point-of-entry disinfection unit, which can use chlorine, ultraviolet light, or ozone.

The Centers for Disease Control and Prevention (CDC) suggests other actions that you may take to
prevent E. coli infection.  These include:
  • Avoid swallowing lake or pool water while swimming.
  • Thoroughly cook ground beef and avoid unpasteurized milk.  
  • Make sure that persons with diarrhea, especially children, wash their hands carefully with soap
    after bowel movements to reduce the risk of spreading infection, and that persons wash hands
    after changing soiled diapers. Anyone with a diarrheal illness should avoid swimming in public
    pools or lakes, sharing baths with others, and preparing food for others.  
  • Cook all ground beef and hamburger thoroughly. Because ground beef can turn brown before
    disease-causing bacteria are killed, use a digital instant-read meat thermometer to ensure
    thorough cooking. Ground beef should be cooked until a thermometer inserted into several
    parts of the patty, including the thickest part, reads at least 160º F. Persons who cook ground
    beef without using a thermometer can decrease their risk of illness by not eating ground beef
    patties that are still pink in the middle.  
  • If you are served an undercooked hamburger or other ground beef product in a restaurant,
    send it back for further cooking. You may want to ask for a new bun and a clean plate, too.  
  • Avoid spreading harmful bacteria in your kitchen. Keep raw meat separate from ready-to-eat
    foods. Wash hands, counters, and utensils with hot soapy water after they touch raw meat.
    Never place cooked hamburgers or ground beef on the unwashed plate that held raw patties.
    Wash meat thermometers in between tests of patties that require further cooking.  
  • Drink only pasteurized milk, juice, or cider. Commercial juice with an extended shelf-life that is
    sold at room temperature (e.g. juice in cardboard boxes, vacuum sealed juice in glass
    containers) has been pasteurized, although this is generally not indicated on the label. Juice
    concentrates are also heated sufficiently to kill pathogens.  
  • Wash fruits and vegetables thoroughly, especially those that will not be cooked. Children under
    5 years of age, immunocompromised persons, and the elderly should avoid eating alfalfa
    sprouts until their safety can be assured. Methods to decontaminate alfalfa seeds and sprouts
    are being investigated.  

Will a water filter work to keep E. coli out of my water?
Most in-home filters will not.  EPA recommends that you boil your water if you are concerned about its
Electron micrograph of
a cluster of E. coli
bacteria - click to